Inhalt des Symposiums ist eine Übersicht und Diskussion über den aktuellen Stand und die Perspektiven der Integration von Metagenom- und NGS-Techniken in die mikrobiologische Diagnostik inklusive des Nutzens von Mikrobiom-Analysen mittels Next-Gen-basierter Technologien. Der aktuelle und potentielle Wert dieser Techniken, auch im Vergleich zu konventioneller Diagnostik, wird in einer Panel-Diskussion kritisch hinterfragt.
09:10 Uhr
V-04:
Next generation sequencing: first diagnostic one-stop shop in personalised clinical microbiology
Prof John Rossen | University of Groningen | Netherlands
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Prof John Rossen | University of Groningen | Netherlands
Current molecular diagnostics of human pathogens provide limited information that is often not sufficient for outbreak and transmission investigation. In addition, when used for detection of pathogens often a biased approach is used. In this presentation an overview of the use of next generation sequencing in clinical microbiology and infection prevention will be given. Next generation sequencing (NGS) determines the DNA sequence of a complete microbial, viral or fungal genome in a single sequence run, and from these data, information on resistance and virulence, as well as information for typing is obtained, useful for outbreak investigation. The obtained genome data can be further used for the development of an outbreak-specific screening test. Like every new technology adopted in microbiology, the integration of NGS into clinical and routine workflows must be carefully managed. As the microbiology laboratories have to adhere to various national and international regulations and criteria for their accreditation, quality control issues for using WGS in microbiology, including the importance of proficiency testing, are presented. In addition, applications of NGS in the clinical setting are discussed, such as outbreak management, molecular case finding, characterization and surveillance of pathogens, rapid identification of bacteria using the 16S-23S rRNA region, taxonomy, and metagenomics approaches on clinical samples. Finally, we share our vision on the use of NGS in personalised microbiology in the near future, pointing out specific requirements.
09:37 Uhr
Round Table Discussion