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FV 22:
Effect of Lactobacillus rhamnosus on emotional behavior in high-fat diet fed mice
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Autor:innen:
M. Schell (Nuthetal, DE)
K. Wardelmann (Nuthetal, DE)
R. Hauffe (Nuthetal, DE)
T. Flore (Nuthetal, DE)
M. Rath (Nuthetal, DE)
A. Kleinridders (Nuthetal, DE)
The prevalence of depression and associated mood disorders is higher in diabetic patients compared to healthy humans. Contributing factors for this association might be deteriorated dopamine signaling, increased inflammation or an overactivation of the hypothalamus-pituitary-adrenal axis. These factors can be modulated by the gut-brain axis through alterations of the gut microbiome, for instance via excessive energy intake. In line, intake of probiotics has been shown to improve mood and metabolism, e.g. the application of Lactobacilli is associated with metabolic improvement and reduced anxiety- and depression-related behavior. Yet, the therapeutic role of Lactobacillus rhamnosus ATCC 53103 (53103) in a model of high-fat diet (HFD) induced mood disorders is unknown and was investigated in the current study.
Female C57BL/6N mice were fed either a low-fat diet (LFD, 10% kcal from fat) or an HFD (45% kcal from fat) for 6 weeks and following received daily oral gavage of either vehicle or 1x10^8 CFU 53103 for 7 weeks (LFD-vehicle, HFD-vehicle, and HFD-53103). Emotional behavior was assessed using Open Field Test (exploration), Dark-Light Box Test (anxiety), Elevated X Maze (stress-induced anxiety) and Mouse tail Suspension Test (depressive-like behavior).
We show that HFD-fed female mice develop obesity and transient increased glycemia but not hyperinsulinemia. Only 2 weeks of 53103 application is sufficient to alleviate HFD-induced hyperactivity and increased glycemia independent of body weight. Furthermore, 53103 increases time spent in the open arms (Elevated X Maze), and decreased adrenal gland weight in HFD-fed mice. In conclusion, 53103 can decrease HFD-induced mood abnormalities and may improve metabolism.