Autor:innen:
C. Hoffmann (Tübingen, DE)
M. Irmler (Neuherberg, DE)
P. Schneeweiss (Tübingen, DE)
E. Randrianarisoa (Tübingen, DE)
G. Schnauder (Tübingen, DE)
J. Machann (Tübingen, DE)
F. Schick (Tübingen, DE)
A. Fritsche (Tübingen, DE)
M. Heni (Tübingen, DE)
A. Nieß (Tübingen, DE)
M. Hrabě de Angelis (Neuherberg, DE)
A. Peter (Tübingen, DE)
A. Birkenfeld (Tübingen, DE)
H. Häring (Tübingen, DE)
J. Beckers (Neuherberg, DE)
A. Böhm (Tübingen, DE)
C. Weigert (Tübingen, DE)
Acute and chronic exercise regulates adaptation of skeletal muscle by changing the transcriptome, but less is known about exercise effects in adipose tissue, especially in humans. Our aim was to characterize the transcriptomic response of adipose tissue to an acute exercise bout and to training.
25 overweight (BMI>27), sedentary but healthy adults underwent an 8-week supervised exercise intervention consisting of 20 sessions combined bike and treadmill exercise (30 min each, 80% individual VO2peak). Before and after intervention resting biopsies were taken from abdominal subcutaneous adipose tissue. Acute exercise biopsies were drawn after the first and last bicycle exercise bout. Transcriptome profiling was done by microarray, statistical evaluation by the limma-t-test.
The first acute exercise bout changed the abundance of 357 transcripts, with 136 transcripts showing a comparable regulation after both, the first and last acute exercise bout (p < 0.01, FC > 1.2). Enriched pathway analysis indicates downregulation of lipid synthesis and inflammation. Among adipokines, ANGPTL8 was 4-fold reduced after both acute exercise bouts. Intervention effects were generally less, with 163 transcripts changed, despite the intervention significantly decreasing BMI and adipose tissue volume while increasing VO2peak and aerobic threshold. No indication for beiging, e.g. upregulation of UCP1, was detected. Comparison with the response of transcripts in skeletal muscle biopsies revealed only little overlap in acutely (10%) or chronically (4%) regulated transcripts between tissues.
Changes in the transcriptome of adipose tissue after acute exercise and training suggest regulation of metabolic pathways and in paracrine/endocrine communication pointing towards a dynamic inter-organ crosstalk.