Authors:
H. Flores-Aguilar (México City, MX)
A. Rodriguez (México City, MX)
B. González (México City, MX)
A. Delgado (México City, MX)
A. Munguia (México City, MX)
C. Gorodezky (Mexico City. MEXICO, MX)
STRs are versatile and informative genetic markers that have facilitated a great understanding of the genetic diversity present in natural populations, being a common tool in forensics, paternity and anthropological studies. Data collection is conducted based on many populations and allele frequency (AF) must be established in making an identification or any study. Mexican Mestizos are of mixed descent. Their ancestry is the result of admixture between the indigenous people of Asian ancestry that inhabited the country, different Europeans, and to a lesser extent, Africans. The aim of the study was to describe the population genetics in Mexico City and Highlands, by using the PowerPlex21 & GenePrint 24(Promega) & Global filer & Identifiler (Thermo Fisher Sci.) systems. We investigated 24 autosomal STRs in genomic DNA extracted from blood samples of 721 healthy Mexican Mestizos (57.4% males; 42.6% females). The analysis was performed with a 3500 Genetic Analyzer (Thermo Fisher Sci.). The genotyping process was done with the Gene Mapper ID-X v1.4 software. We compared the frequency with other studies in Mexicans from different regions. Heterozygosity (HE), Linkage disequilibrium (LD); Hardy-Weinberg equilibrium (HWE), Power of Discrimination (PD), Match Probability (MP) and Power of Exclusion (PE) were estimated with the PowerStat software. A total of 339 alleles at the 24 STR loci were found with their corresponding AF, ranging 0.069-50.704. These STRs are highly polymorphic being the most diverse FGA, D18551, Penta-E, D21S11, D6S1043 and SE33 (30-18 alleles). Penta E showed the highest PD(0.985) and SE33,the highest PE (0.947). Concordantly, these STR loci showed the highest heterozygosity together with D6S1043; one allele was not in HWE at; vWA, D8S1179, Penta D, D12S391, D19S433, D16S539; two alleles at TH01, Penta E, D21S11; 3 at D18S51, FGA, CSF1P0, since they were rare alleles. LD displayed no association between paired loci. Our data are concordant with those shown in Monterrey (North Mexico) but show different AF from them & those found in other Mexico regions. The most frequent alleles >20% were at; D21S11; D6S1043; vWA;TH01;CSF1P0; D16S539; D8S1179; D3S1358; D19S433, D2S391; D7S820. STR loci provide highly informative polymorphic data for population genetics, paternity testing and forensic identification.