Authors:
E. Kuzmich (St.-Petersburg, RU)
O. Makarenko (Saint-Petersburg, RU)
A. Alynskiy (Saint-Petersburg, RU)
V. Ermolina (Saint-Petersburg, RU)
S. Merzlyakova (Saint-Petersburg, RU)
N. Ivanova (Saint-Petersburg, RU)
L. Zubarovskaya (Saint-Petersburg, RU)
B. Afanasyev (Saint-Petersburg, RU)
In December 2016 the Database of the Bone Marrow Donors Registry of Pavlov First Saint-Petersburg State Medical University included information about HLA phenotypes of 13000 volunteer donors. High resolution HLA typing by monoallelic Sanger-sequencing was carried out for 1000 donors. Data analysis was performed by Arlequin program version 3.5. The expectation–maximization (EM) algorithm was used for the determination of five-locus haplotype frequencies. In the studied group of donors the most common alleles were: HLA-A*02:01 – 23.9%, HLA-A*03:01 – 13.9%, HLA-A*01:01 – 10.9%, A*24:02 – 10.2%, HLA-A*11:01 – 4.6%, HLA-A*25:01 – 4.2%. HLA-B*07:02 – 11.6%, HLA-B*08:01 – 6.7%, HLA-B*18:01 – 5.1%, HLA-B*13:02 – 4.9%, HLA-B*35:01 – 4.9%, HLA-B*44:02 – 4.7%. HLA-C*07:02 – 13.1%, HLA-C*07:01 – 10.7%, HLA-C*04:01 – 9.3%, HLA-C*06:02 – 9.0%, HLA-C*12:03 – 8.3%, HLA-C*02:02 – 5.5%. HLA-DRB1*07:01 – 13.2%, DRB1*15:01 – 11.5%, HLA-DRB1*01:01 – 10.2%, HLA-DRB1*03:01 – 8.2%, HLA- DRB1*13:01 – 5.2%, DRB1*16:01 – 4.4%. HLA-DQB1*03:01 – 14.6%, HLA-DQB1*02:01 – 13.9%, HLA-DQB1*05:01 – 11.5%, HLA- DQB1*06:02 – 10.7%, HLA- DQB1*03:02 – 6.2%, HLA- DQB1*06:03 – 5.8%. The most frequent five-locus high resolution HLA haplotypes were: A*01:01-B*08:01-C*07:01-DRB1*03:01-DQB1*02:01 (3.4%); A*03:01-B*07:02-C*07:02-DRB1*15:01-DQB1*06:02 (3.1%); A*03:01-B*35:01-C*04:01-DRB1*01:01-DQB1*05:01 (1.8%); A*02:01-B*07:02-C*07:02-DRB1*15:01-DQB1*06:02 (1.3%); A*25:01-B*18:01-C*12:03-DRB1*15:01-DQB1*06:02 (1.03%); A*02:01-B*13:02-C*06:02-DRB1*07:01-DQB1*02:01 (1.01%). This study reports the most frequent HLA alleles and five-locus high resolution haplotypes in the donors of Bone Marrow Donors Registry of Pavlov First Saint-Petersburg State Medical University.